Virus:Article Title: Fn14 is an activity-dependent, Bmal1-regulated cytokine receptor that induces rod-like microglia and restricts neuronal activity in vivo.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken anti Iba1 Synaptic Systems Cat# 234009; RRID: AB_2891282 Rabbit anti vGluT1 Invitrogen Cat# 48-2400; RRID: AB_2533843 Mouse anti vGaT Synaptic Systems Cat# 131001; AB_2315584 Alexafluor 488 goat anti rabbit Abcam Cat# 150077; RRID: AB_2630356 Alexafluor 555 rabbit anti goat Thermofisher Cat# A21428; RRID: AB_2535849 Alexafluor 488 chicken anti rabbit Synaptic Systems Rabbit anti Fn14 Cell Signaling Cat# 4403s; RRID: AB_10693941 Rabbit anti HA Cell Signaling Cat# 3724; RRID: AB_1549585 Rabbit anti vGluT1 Invitrogen Cat# 48-2400; RRID: AB_2533843 Alexafluor 555 donkey anti rabbit Invitrogen Cat# A31572; RRID: AB_162543 Alexafluor 647 donkey anti chicken Jackson Immuno Research Cat# 703-605-155; RRID: AB_2340379 Chicken anti Homer1b/c Synaptic Systems Cat# 160026; RRID: AB_2713982 Bacterial and virus strains pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 James M. Wilson Addgene 100834 eBFP2 (eBFP2)_CD Iverson et al 2016 Addgene 66034 pAAV-hSyn-EGFP Bryan Roth Addgene 50465 AAV9-hSyn-eBFP2 Made in house NA AAV9-hSyn-HA-Fn14-IRES-eGFP Made in house NA pENN.AAV.hSyn.HI.eGFPCre.WPRE.SV40 James M. Wilson Addgene 105540 Chemicals, peptides, and recombinant proteins Kainate Sigma-Aldrich Cat# K0250-50MG EcoRI-HF New England BioLabs Cat# NEB:R3101s BamHI-HF New England BioLabs Cat# NEB:R3136s Gibson reaction New England BioLabs Cat# NEB: E2621S T4 ligase New England BioLabs Cat# NEB:M0202S Pentylenetetrazole Sigma-Aldrich Cat# P6500 ViaStain AOPI Nexcelom Cat# CS2-0106-5mL RIPA lysis buffer Thermoscientific Cat# 89900 Protease inhibitor cocktail Thermoscientific Cat# 78429 Phosphotase inhibitor cocktail Thermoscientific Cat# 78428 Sodium n-dodecyl sulfate (SDS) Thermoscientific Cat# 28364 1M Tris-HCI, pH 8.0 Thermoscientific Cat# 15568025 NuPAGE TM LDS Sample Buffer (4X) Thermoscientific Cat# NP0007 XT MES Running Buffer Bio-Rad Cat# 1610789 Sodium Deoxycholate Detergent Thermoscientific Cat# 89904 Critical commercial assays RNAscope multiplex fluorescent V2 assay Advanced Cell Diagnostics [ACD], Biotechne Cat# 323110 Quantitative multiplexed detection of cytokines, chemokines, and growth factors Eve Technologies Corporation NA GEM-X Universal Gene Expression v4 kit 10X Genomics Cat# 1000691 TransAM® AP-1 Family Activ Motif Cat# 44296 (Continued on next page) 18 Cell Reports 45, 116926, February 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Pollina et al., (2023) RNA sequencing data Pollina et al. 27 GEO ID# GSE175965 Yap et al., (2021) RNA sequencing data Yap et al. 28 GEO ID# GSE158843 Single nuclear RNA sequencing data This paper GEO accession# GSE315059 Yap et al., (2021) RNA sequencing data Yap et al. 28 GEO ID# GSE158843 Experimental models: Organisms/strains Mouse:C57Bl/6J The Jackson Laboratory Cat# JAX:000664 Mouse:B6.Tnfrsf12a tm1(KO)Biogen Dr. Linda Burkly, Biogen NA mouse:B6.129S4(CG)-Bmal1 tm1Weit /J The Jackson Laboratory Cat# JAX:007668 Oligonucleotides gaaggtaccggatccgccacGAT GGTGAGCAAGGGCGAG Millipore Sigma NA tatcgataagcttgatatcg CAGCGAGTCAGTGAGCGAG Millipore Sigma NA ACAAAAGCTAGCgccaccAT GTACCCATACGATGTTCCAGAT TACGCTAtggcttcggcttggccgc ggtctctgccgcagatcctcgtg ttgggattcggcttggtgttgatgcgcgccg cggccggggagcaagcaccaggcacctc cccatgctctagcggcagctcctggagcgcg gacctcgacaagtgcatggactgcgcttctt gtccagcgcgaccacacagcgac ttctgcctgggatgcgccgcagcacctcctgcccac ttcaggctactgtggcccattctg gggggcgctcttagtctggtcctggttttgg cgctggtttctagtttcctggtctggagaagatgc cgccggagagaaaagtttactacccccat agaggagactggtggagagggctgcc caggtgtggcactgatccagtgaccattaacgcgc gaccagcttgatatcgaattggcccct ctccctcccccccccctaacgtta ctggccgaagccgcttggaataa ggccggtgtgcgtttg tctatatgttattttccaccatattgc cgtcttttggcaatgtgagggcccggaa acctggccctgtcttcttgacgagcattcctaggg gtctttcccctctcgccaaaggaatg caaggtctgttgaatgtcgtgaagg aagcagttcctctggaagcttcttga agacaaacaacgt ctgtagcgaccctttgcaggcagcg gaaccccccacctggcgacaggt gcctctgcggccaaaagccacgtg tataagatacacctgcaa aggcggcacaaccccagtgcca cgttgtgagttggatagttgtggaaaga gtcaaatggctctcctcaagcgtatt caacaaggggctga aggatgcccagaaggtaccccattg tatgggatctgatctggggcctcggtaca catgctttacatgtgtttagtcgaggttaaaaaaac gtctaggccccccgaaccacggggac gtggttttcctttgaaaaacacgatgataat atggccacaaccatggtgagcaagggcgaggag ctgttcaccggggtggtgcccatcc GeneScript NA (Continued on next page) Cell Reports 45, 116926, February 24, 2026 19
Recombinant:Article Title: Fn14 is an activity-dependent, Bmal1-regulated cytokine receptor that induces rod-like microglia and restricts neuronal activity in vivo.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken anti Iba1 Synaptic Systems Cat# 234009; RRID: AB_2891282 Rabbit anti vGluT1 Invitrogen Cat# 48-2400; RRID: AB_2533843 Mouse anti vGaT Synaptic Systems Cat# 131001; AB_2315584 Alexafluor 488 goat anti rabbit Abcam Cat# 150077; RRID: AB_2630356 Alexafluor 555 rabbit anti goat Thermofisher Cat# A21428; RRID: AB_2535849 Alexafluor 488 chicken anti rabbit Synaptic Systems Rabbit anti Fn14 Cell Signaling Cat# 4403s; RRID: AB_10693941 Rabbit anti HA Cell Signaling Cat# 3724; RRID: AB_1549585 Rabbit anti vGluT1 Invitrogen Cat# 48-2400; RRID: AB_2533843 Alexafluor 555 donkey anti rabbit Invitrogen Cat# A31572; RRID: AB_162543 Alexafluor 647 donkey anti chicken Jackson Immuno Research Cat# 703-605-155; RRID: AB_2340379 Chicken anti Homer1b/c Synaptic Systems Cat# 160026; RRID: AB_2713982 Bacterial and virus strains pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 James M. Wilson Addgene 100834 eBFP2 (eBFP2)_CD Iverson et al 2016 Addgene 66034 pAAV-hSyn-EGFP Bryan Roth Addgene 50465 AAV9-hSyn-eBFP2 Made in house NA AAV9-hSyn-HA-Fn14-IRES-eGFP Made in house NA pENN.AAV.hSyn.HI.eGFPCre.WPRE.SV40 James M. Wilson Addgene 105540 Chemicals, peptides, and recombinant proteins Kainate Sigma-Aldrich Cat# K0250-50MG EcoRI-HF New England BioLabs Cat# NEB:R3101s BamHI-HF New England BioLabs Cat# NEB:R3136s Gibson reaction New England BioLabs Cat# NEB: E2621S T4 ligase New England BioLabs Cat# NEB:M0202S Pentylenetetrazole Sigma-Aldrich Cat# P6500 ViaStain AOPI Nexcelom Cat# CS2-0106-5mL RIPA lysis buffer Thermoscientific Cat# 89900 Protease inhibitor cocktail Thermoscientific Cat# 78429 Phosphotase inhibitor cocktail Thermoscientific Cat# 78428 Sodium n-dodecyl sulfate (SDS) Thermoscientific Cat# 28364 1M Tris-HCI, pH 8.0 Thermoscientific Cat# 15568025 NuPAGE TM LDS Sample Buffer (4X) Thermoscientific Cat# NP0007 XT MES Running Buffer Bio-Rad Cat# 1610789 Sodium Deoxycholate Detergent Thermoscientific Cat# 89904 Critical commercial assays RNAscope multiplex fluorescent V2 assay Advanced Cell Diagnostics [ACD], Biotechne Cat# 323110 Quantitative multiplexed detection of cytokines, chemokines, and growth factors Eve Technologies Corporation NA GEM-X Universal Gene Expression v4 kit 10X Genomics Cat# 1000691 TransAM® AP-1 Family Activ Motif Cat# 44296 (Continued on next page) 18 Cell Reports 45, 116926, February 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Pollina et al., (2023) RNA sequencing data Pollina et al. 27 GEO ID# GSE175965 Yap et al., (2021) RNA sequencing data Yap et al. 28 GEO ID# GSE158843 Single nuclear RNA sequencing data This paper GEO accession# GSE315059 Yap et al., (2021) RNA sequencing data Yap et al. 28 GEO ID# GSE158843 Experimental models: Organisms/strains Mouse:C57Bl/6J The Jackson Laboratory Cat# JAX:000664 Mouse:B6.Tnfrsf12a tm1(KO)Biogen Dr. Linda Burkly, Biogen NA mouse:B6.129S4(CG)-Bmal1 tm1Weit /J The Jackson Laboratory Cat# JAX:007668 Oligonucleotides gaaggtaccggatccgccacGAT GGTGAGCAAGGGCGAG Millipore Sigma NA tatcgataagcttgatatcg CAGCGAGTCAGTGAGCGAG Millipore Sigma NA ACAAAAGCTAGCgccaccAT GTACCCATACGATGTTCCAGAT TACGCTAtggcttcggcttggccgc ggtctctgccgcagatcctcgtg ttgggattcggcttggtgttgatgcgcgccg cggccggggagcaagcaccaggcacctc cccatgctctagcggcagctcctggagcgcg gacctcgacaagtgcatggactgcgcttctt gtccagcgcgaccacacagcgac ttctgcctgggatgcgccgcagcacctcctgcccac ttcaggctactgtggcccattctg gggggcgctcttagtctggtcctggttttgg cgctggtttctagtttcctggtctggagaagatgc cgccggagagaaaagtttactacccccat agaggagactggtggagagggctgcc caggtgtggcactgatccagtgaccattaacgcgc gaccagcttgatatcgaattggcccct ctccctcccccccccctaacgtta ctggccgaagccgcttggaataa ggccggtgtgcgtttg tctatatgttattttccaccatattgc cgtcttttggcaatgtgagggcccggaa acctggccctgtcttcttgacgagcattcctaggg gtctttcccctctcgccaaaggaatg caaggtctgttgaatgtcgtgaagg aagcagttcctctggaagcttcttga agacaaacaacgt ctgtagcgaccctttgcaggcagcg gaaccccccacctggcgacaggt gcctctgcggccaaaagccacgtg tataagatacacctgcaa aggcggcacaaccccagtgcca cgttgtgagttggatagttgtggaaaga gtcaaatggctctcctcaagcgtatt caacaaggggctga aggatgcccagaaggtaccccattg tatgggatctgatctggggcctcggtaca catgctttacatgtgtttagtcgaggttaaaaaaac gtctaggccccccgaaccacggggac gtggttttcctttgaaaaacacgatgataat atggccacaaccatggtgagcaagggcgaggag ctgttcaccggggtggtgcccatcc GeneScript NA (Continued on next page) Cell Reports 45, 116926, February 24, 2026 19
Lysis:Article Title: Fn14 is an activity-dependent, Bmal1-regulated cytokine receptor that induces rod-like microglia and restricts neuronal activity in vivo.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken anti Iba1 Synaptic Systems Cat# 234009; RRID: AB_2891282 Rabbit anti vGluT1 Invitrogen Cat# 48-2400; RRID: AB_2533843 Mouse anti vGaT Synaptic Systems Cat# 131001; AB_2315584 Alexafluor 488 goat anti rabbit Abcam Cat# 150077; RRID: AB_2630356 Alexafluor 555 rabbit anti goat Thermofisher Cat# A21428; RRID: AB_2535849 Alexafluor 488 chicken anti rabbit Synaptic Systems Rabbit anti Fn14 Cell Signaling Cat# 4403s; RRID: AB_10693941 Rabbit anti HA Cell Signaling Cat# 3724; RRID: AB_1549585 Rabbit anti vGluT1 Invitrogen Cat# 48-2400; RRID: AB_2533843 Alexafluor 555 donkey anti rabbit Invitrogen Cat# A31572; RRID: AB_162543 Alexafluor 647 donkey anti chicken Jackson Immuno Research Cat# 703-605-155; RRID: AB_2340379 Chicken anti Homer1b/c Synaptic Systems Cat# 160026; RRID: AB_2713982 Bacterial and virus strains pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 James M. Wilson Addgene 100834 eBFP2 (eBFP2)_CD Iverson et al 2016 Addgene 66034 pAAV-hSyn-EGFP Bryan Roth Addgene 50465 AAV9-hSyn-eBFP2 Made in house NA AAV9-hSyn-HA-Fn14-IRES-eGFP Made in house NA pENN.AAV.hSyn.HI.eGFPCre.WPRE.SV40 James M. Wilson Addgene 105540 Chemicals, peptides, and recombinant proteins Kainate Sigma-Aldrich Cat# K0250-50MG EcoRI-HF New England BioLabs Cat# NEB:R3101s BamHI-HF New England BioLabs Cat# NEB:R3136s Gibson reaction New England BioLabs Cat# NEB: E2621S T4 ligase New England BioLabs Cat# NEB:M0202S Pentylenetetrazole Sigma-Aldrich Cat# P6500 ViaStain AOPI Nexcelom Cat# CS2-0106-5mL RIPA lysis buffer Thermoscientific Cat# 89900 Protease inhibitor cocktail Thermoscientific Cat# 78429 Phosphotase inhibitor cocktail Thermoscientific Cat# 78428 Sodium n-dodecyl sulfate (SDS) Thermoscientific Cat# 28364 1M Tris-HCI, pH 8.0 Thermoscientific Cat# 15568025 NuPAGE TM LDS Sample Buffer (4X) Thermoscientific Cat# NP0007 XT MES Running Buffer Bio-Rad Cat# 1610789 Sodium Deoxycholate Detergent Thermoscientific Cat# 89904 Critical commercial assays RNAscope multiplex fluorescent V2 assay Advanced Cell Diagnostics [ACD], Biotechne Cat# 323110 Quantitative multiplexed detection of cytokines, chemokines, and growth factors Eve Technologies Corporation NA GEM-X Universal Gene Expression v4 kit 10X Genomics Cat# 1000691 TransAM® AP-1 Family Activ Motif Cat# 44296 (Continued on next page) 18 Cell Reports 45, 116926, February 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Pollina et al., (2023) RNA sequencing data Pollina et al. 27 GEO ID# GSE175965 Yap et al., (2021) RNA sequencing data Yap et al. 28 GEO ID# GSE158843 Single nuclear RNA sequencing data This paper GEO accession# GSE315059 Yap et al., (2021) RNA sequencing data Yap et al. 28 GEO ID# GSE158843 Experimental models: Organisms/strains Mouse:C57Bl/6J The Jackson Laboratory Cat# JAX:000664 Mouse:B6.Tnfrsf12a tm1(KO)Biogen Dr. Linda Burkly, Biogen NA mouse:B6.129S4(CG)-Bmal1 tm1Weit /J The Jackson Laboratory Cat# JAX:007668 Oligonucleotides gaaggtaccggatccgccacGAT GGTGAGCAAGGGCGAG Millipore Sigma NA tatcgataagcttgatatcg CAGCGAGTCAGTGAGCGAG Millipore Sigma NA ACAAAAGCTAGCgccaccAT GTACCCATACGATGTTCCAGAT TACGCTAtggcttcggcttggccgc ggtctctgccgcagatcctcgtg ttgggattcggcttggtgttgatgcgcgccg cggccggggagcaagcaccaggcacctc cccatgctctagcggcagctcctggagcgcg gacctcgacaagtgcatggactgcgcttctt gtccagcgcgaccacacagcgac ttctgcctgggatgcgccgcagcacctcctgcccac ttcaggctactgtggcccattctg gggggcgctcttagtctggtcctggttttgg cgctggtttctagtttcctggtctggagaagatgc cgccggagagaaaagtttactacccccat agaggagactggtggagagggctgcc caggtgtggcactgatccagtgaccattaacgcgc gaccagcttgatatcgaattggcccct ctccctcccccccccctaacgtta ctggccgaagccgcttggaataa ggccggtgtgcgtttg tctatatgttattttccaccatattgc cgtcttttggcaatgtgagggcccggaa acctggccctgtcttcttgacgagcattcctaggg gtctttcccctctcgccaaaggaatg caaggtctgttgaatgtcgtgaagg aagcagttcctctggaagcttcttga agacaaacaacgt ctgtagcgaccctttgcaggcagcg gaaccccccacctggcgacaggt gcctctgcggccaaaagccacgtg tataagatacacctgcaa aggcggcacaaccccagtgcca cgttgtgagttggatagttgtggaaaga gtcaaatggctctcctcaagcgtatt caacaaggggctga aggatgcccagaaggtaccccattg tatgggatctgatctggggcctcggtaca catgctttacatgtgtttagtcgaggttaaaaaaac gtctaggccccccgaaccacggggac gtggttttcctttgaaaaacacgatgataat atggccacaaccatggtgagcaagggcgaggag ctgttcaccggggtggtgcccatcc GeneScript NA (Continued on next page) Cell Reports 45, 116926, February 24, 2026 19
Protease Inhibitor:Article Title: Fn14 is an activity-dependent, Bmal1-regulated cytokine receptor that induces rod-like microglia and restricts neuronal activity in vivo.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken anti Iba1 Synaptic Systems Cat# 234009; RRID: AB_2891282 Rabbit anti vGluT1 Invitrogen Cat# 48-2400; RRID: AB_2533843 Mouse anti vGaT Synaptic Systems Cat# 131001; AB_2315584 Alexafluor 488 goat anti rabbit Abcam Cat# 150077; RRID: AB_2630356 Alexafluor 555 rabbit anti goat Thermofisher Cat# A21428; RRID: AB_2535849 Alexafluor 488 chicken anti rabbit Synaptic Systems Rabbit anti Fn14 Cell Signaling Cat# 4403s; RRID: AB_10693941 Rabbit anti HA Cell Signaling Cat# 3724; RRID: AB_1549585 Rabbit anti vGluT1 Invitrogen Cat# 48-2400; RRID: AB_2533843 Alexafluor 555 donkey anti rabbit Invitrogen Cat# A31572; RRID: AB_162543 Alexafluor 647 donkey anti chicken Jackson Immuno Research Cat# 703-605-155; RRID: AB_2340379 Chicken anti Homer1b/c Synaptic Systems Cat# 160026; RRID: AB_2713982 Bacterial and virus strains pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 James M. Wilson Addgene 100834 eBFP2 (eBFP2)_CD Iverson et al 2016 Addgene 66034 pAAV-hSyn-EGFP Bryan Roth Addgene 50465 AAV9-hSyn-eBFP2 Made in house NA AAV9-hSyn-HA-Fn14-IRES-eGFP Made in house NA pENN.AAV.hSyn.HI.eGFPCre.WPRE.SV40 James M. Wilson Addgene 105540 Chemicals, peptides, and recombinant proteins Kainate Sigma-Aldrich Cat# K0250-50MG EcoRI-HF New England BioLabs Cat# NEB:R3101s BamHI-HF New England BioLabs Cat# NEB:R3136s Gibson reaction New England BioLabs Cat# NEB: E2621S T4 ligase New England BioLabs Cat# NEB:M0202S Pentylenetetrazole Sigma-Aldrich Cat# P6500 ViaStain AOPI Nexcelom Cat# CS2-0106-5mL RIPA lysis buffer Thermoscientific Cat# 89900 Protease inhibitor cocktail Thermoscientific Cat# 78429 Phosphotase inhibitor cocktail Thermoscientific Cat# 78428 Sodium n-dodecyl sulfate (SDS) Thermoscientific Cat# 28364 1M Tris-HCI, pH 8.0 Thermoscientific Cat# 15568025 NuPAGE TM LDS Sample Buffer (4X) Thermoscientific Cat# NP0007 XT MES Running Buffer Bio-Rad Cat# 1610789 Sodium Deoxycholate Detergent Thermoscientific Cat# 89904 Critical commercial assays RNAscope multiplex fluorescent V2 assay Advanced Cell Diagnostics [ACD], Biotechne Cat# 323110 Quantitative multiplexed detection of cytokines, chemokines, and growth factors Eve Technologies Corporation NA GEM-X Universal Gene Expression v4 kit 10X Genomics Cat# 1000691 TransAM® AP-1 Family Activ Motif Cat# 44296 (Continued on next page) 18 Cell Reports 45, 116926, February 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Pollina et al., (2023) RNA sequencing data Pollina et al. 27 GEO ID# GSE175965 Yap et al., (2021) RNA sequencing data Yap et al. 28 GEO ID# GSE158843 Single nuclear RNA sequencing data This paper GEO accession# GSE315059 Yap et al., (2021) RNA sequencing data Yap et al. 28 GEO ID# GSE158843 Experimental models: Organisms/strains Mouse:C57Bl/6J The Jackson Laboratory Cat# JAX:000664 Mouse:B6.Tnfrsf12a tm1(KO)Biogen Dr. Linda Burkly, Biogen NA mouse:B6.129S4(CG)-Bmal1 tm1Weit /J The Jackson Laboratory Cat# JAX:007668 Oligonucleotides gaaggtaccggatccgccacGAT GGTGAGCAAGGGCGAG Millipore Sigma NA tatcgataagcttgatatcg CAGCGAGTCAGTGAGCGAG Millipore Sigma NA ACAAAAGCTAGCgccaccAT GTACCCATACGATGTTCCAGAT TACGCTAtggcttcggcttggccgc ggtctctgccgcagatcctcgtg ttgggattcggcttggtgttgatgcgcgccg cggccggggagcaagcaccaggcacctc cccatgctctagcggcagctcctggagcgcg gacctcgacaagtgcatggactgcgcttctt gtccagcgcgaccacacagcgac ttctgcctgggatgcgccgcagcacctcctgcccac ttcaggctactgtggcccattctg gggggcgctcttagtctggtcctggttttgg cgctggtttctagtttcctggtctggagaagatgc cgccggagagaaaagtttactacccccat agaggagactggtggagagggctgcc caggtgtggcactgatccagtgaccattaacgcgc gaccagcttgatatcgaattggcccct ctccctcccccccccctaacgtta ctggccgaagccgcttggaataa ggccggtgtgcgtttg tctatatgttattttccaccatattgc cgtcttttggcaatgtgagggcccggaa acctggccctgtcttcttgacgagcattcctaggg gtctttcccctctcgccaaaggaatg caaggtctgttgaatgtcgtgaagg aagcagttcctctggaagcttcttga agacaaacaacgt ctgtagcgaccctttgcaggcagcg gaaccccccacctggcgacaggt gcctctgcggccaaaagccacgtg tataagatacacctgcaa aggcggcacaaccccagtgcca cgttgtgagttggatagttgtggaaaga gtcaaatggctctcctcaagcgtatt caacaaggggctga aggatgcccagaaggtaccccattg tatgggatctgatctggggcctcggtaca catgctttacatgtgtttagtcgaggttaaaaaaac gtctaggccccccgaaccacggggac gtggttttcctttgaaaaacacgatgataat atggccacaaccatggtgagcaagggcgaggag ctgttcaccggggtggtgcccatcc GeneScript NA (Continued on next page) Cell Reports 45, 116926, February 24, 2026 19
RNAscope:Article Title: Fn14 is an activity-dependent, Bmal1-regulated cytokine receptor that induces rod-like microglia and restricts neuronal activity in vivo.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken anti Iba1 Synaptic Systems Cat# 234009; RRID: AB_2891282 Rabbit anti vGluT1 Invitrogen Cat# 48-2400; RRID: AB_2533843 Mouse anti vGaT Synaptic Systems Cat# 131001; AB_2315584 Alexafluor 488 goat anti rabbit Abcam Cat# 150077; RRID: AB_2630356 Alexafluor 555 rabbit anti goat Thermofisher Cat# A21428; RRID: AB_2535849 Alexafluor 488 chicken anti rabbit Synaptic Systems Rabbit anti Fn14 Cell Signaling Cat# 4403s; RRID: AB_10693941 Rabbit anti HA Cell Signaling Cat# 3724; RRID: AB_1549585 Rabbit anti vGluT1 Invitrogen Cat# 48-2400; RRID: AB_2533843 Alexafluor 555 donkey anti rabbit Invitrogen Cat# A31572; RRID: AB_162543 Alexafluor 647 donkey anti chicken Jackson Immuno Research Cat# 703-605-155; RRID: AB_2340379 Chicken anti Homer1b/c Synaptic Systems Cat# 160026; RRID: AB_2713982 Bacterial and virus strains pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 James M. Wilson Addgene 100834 eBFP2 (eBFP2)_CD Iverson et al 2016 Addgene 66034 pAAV-hSyn-EGFP Bryan Roth Addgene 50465 AAV9-hSyn-eBFP2 Made in house NA AAV9-hSyn-HA-Fn14-IRES-eGFP Made in house NA pENN.AAV.hSyn.HI.eGFPCre.WPRE.SV40 James M. Wilson Addgene 105540 Chemicals, peptides, and recombinant proteins Kainate Sigma-Aldrich Cat# K0250-50MG EcoRI-HF New England BioLabs Cat# NEB:R3101s BamHI-HF New England BioLabs Cat# NEB:R3136s Gibson reaction New England BioLabs Cat# NEB: E2621S T4 ligase New England BioLabs Cat# NEB:M0202S Pentylenetetrazole Sigma-Aldrich Cat# P6500 ViaStain AOPI Nexcelom Cat# CS2-0106-5mL RIPA lysis buffer Thermoscientific Cat# 89900 Protease inhibitor cocktail Thermoscientific Cat# 78429 Phosphotase inhibitor cocktail Thermoscientific Cat# 78428 Sodium n-dodecyl sulfate (SDS) Thermoscientific Cat# 28364 1M Tris-HCI, pH 8.0 Thermoscientific Cat# 15568025 NuPAGE TM LDS Sample Buffer (4X) Thermoscientific Cat# NP0007 XT MES Running Buffer Bio-Rad Cat# 1610789 Sodium Deoxycholate Detergent Thermoscientific Cat# 89904 Critical commercial assays RNAscope multiplex fluorescent V2 assay Advanced Cell Diagnostics [ACD], Biotechne Cat# 323110 Quantitative multiplexed detection of cytokines, chemokines, and growth factors Eve Technologies Corporation NA GEM-X Universal Gene Expression v4 kit 10X Genomics Cat# 1000691 TransAM® AP-1 Family Activ Motif Cat# 44296 (Continued on next page) 18 Cell Reports 45, 116926, February 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Pollina et al., (2023) RNA sequencing data Pollina et al. 27 GEO ID# GSE175965 Yap et al., (2021) RNA sequencing data Yap et al. 28 GEO ID# GSE158843 Single nuclear RNA sequencing data This paper GEO accession# GSE315059 Yap et al., (2021) RNA sequencing data Yap et al. 28 GEO ID# GSE158843 Experimental models: Organisms/strains Mouse:C57Bl/6J The Jackson Laboratory Cat# JAX:000664 Mouse:B6.Tnfrsf12a tm1(KO)Biogen Dr. Linda Burkly, Biogen NA mouse:B6.129S4(CG)-Bmal1 tm1Weit /J The Jackson Laboratory Cat# JAX:007668 Oligonucleotides gaaggtaccggatccgccacGAT GGTGAGCAAGGGCGAG Millipore Sigma NA tatcgataagcttgatatcg CAGCGAGTCAGTGAGCGAG Millipore Sigma NA ACAAAAGCTAGCgccaccAT GTACCCATACGATGTTCCAGAT TACGCTAtggcttcggcttggccgc ggtctctgccgcagatcctcgtg ttgggattcggcttggtgttgatgcgcgccg cggccggggagcaagcaccaggcacctc cccatgctctagcggcagctcctggagcgcg gacctcgacaagtgcatggactgcgcttctt gtccagcgcgaccacacagcgac ttctgcctgggatgcgccgcagcacctcctgcccac ttcaggctactgtggcccattctg gggggcgctcttagtctggtcctggttttgg cgctggtttctagtttcctggtctggagaagatgc cgccggagagaaaagtttactacccccat agaggagactggtggagagggctgcc caggtgtggcactgatccagtgaccattaacgcgc gaccagcttgatatcgaattggcccct ctccctcccccccccctaacgtta ctggccgaagccgcttggaataa ggccggtgtgcgtttg tctatatgttattttccaccatattgc cgtcttttggcaatgtgagggcccggaa acctggccctgtcttcttgacgagcattcctaggg gtctttcccctctcgccaaaggaatg caaggtctgttgaatgtcgtgaagg aagcagttcctctggaagcttcttga agacaaacaacgt ctgtagcgaccctttgcaggcagcg gaaccccccacctggcgacaggt gcctctgcggccaaaagccacgtg tataagatacacctgcaa aggcggcacaaccccagtgcca cgttgtgagttggatagttgtggaaaga gtcaaatggctctcctcaagcgtatt caacaaggggctga aggatgcccagaaggtaccccattg tatgggatctgatctggggcctcggtaca catgctttacatgtgtttagtcgaggttaaaaaaac gtctaggccccccgaaccacggggac gtggttttcctttgaaaaacacgatgataat atggccacaaccatggtgagcaagggcgaggag ctgttcaccggggtggtgcccatcc GeneScript NA (Continued on next page) Cell Reports 45, 116926, February 24, 2026 19
Multiplex Assay:Article Title: Fn14 is an activity-dependent, Bmal1-regulated cytokine receptor that induces rod-like microglia and restricts neuronal activity in vivo.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken anti Iba1 Synaptic Systems Cat# 234009; RRID: AB_2891282 Rabbit anti vGluT1 Invitrogen Cat# 48-2400; RRID: AB_2533843 Mouse anti vGaT Synaptic Systems Cat# 131001; AB_2315584 Alexafluor 488 goat anti rabbit Abcam Cat# 150077; RRID: AB_2630356 Alexafluor 555 rabbit anti goat Thermofisher Cat# A21428; RRID: AB_2535849 Alexafluor 488 chicken anti rabbit Synaptic Systems Rabbit anti Fn14 Cell Signaling Cat# 4403s; RRID: AB_10693941 Rabbit anti HA Cell Signaling Cat# 3724; RRID: AB_1549585 Rabbit anti vGluT1 Invitrogen Cat# 48-2400; RRID: AB_2533843 Alexafluor 555 donkey anti rabbit Invitrogen Cat# A31572; RRID: AB_162543 Alexafluor 647 donkey anti chicken Jackson Immuno Research Cat# 703-605-155; RRID: AB_2340379 Chicken anti Homer1b/c Synaptic Systems Cat# 160026; RRID: AB_2713982 Bacterial and virus strains pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 James M. Wilson Addgene 100834 eBFP2 (eBFP2)_CD Iverson et al 2016 Addgene 66034 pAAV-hSyn-EGFP Bryan Roth Addgene 50465 AAV9-hSyn-eBFP2 Made in house NA AAV9-hSyn-HA-Fn14-IRES-eGFP Made in house NA pENN.AAV.hSyn.HI.eGFPCre.WPRE.SV40 James M. Wilson Addgene 105540 Chemicals, peptides, and recombinant proteins Kainate Sigma-Aldrich Cat# K0250-50MG EcoRI-HF New England BioLabs Cat# NEB:R3101s BamHI-HF New England BioLabs Cat# NEB:R3136s Gibson reaction New England BioLabs Cat# NEB: E2621S T4 ligase New England BioLabs Cat# NEB:M0202S Pentylenetetrazole Sigma-Aldrich Cat# P6500 ViaStain AOPI Nexcelom Cat# CS2-0106-5mL RIPA lysis buffer Thermoscientific Cat# 89900 Protease inhibitor cocktail Thermoscientific Cat# 78429 Phosphotase inhibitor cocktail Thermoscientific Cat# 78428 Sodium n-dodecyl sulfate (SDS) Thermoscientific Cat# 28364 1M Tris-HCI, pH 8.0 Thermoscientific Cat# 15568025 NuPAGE TM LDS Sample Buffer (4X) Thermoscientific Cat# NP0007 XT MES Running Buffer Bio-Rad Cat# 1610789 Sodium Deoxycholate Detergent Thermoscientific Cat# 89904 Critical commercial assays RNAscope multiplex fluorescent V2 assay Advanced Cell Diagnostics [ACD], Biotechne Cat# 323110 Quantitative multiplexed detection of cytokines, chemokines, and growth factors Eve Technologies Corporation NA GEM-X Universal Gene Expression v4 kit 10X Genomics Cat# 1000691 TransAM® AP-1 Family Activ Motif Cat# 44296 (Continued on next page) 18 Cell Reports 45, 116926, February 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Pollina et al., (2023) RNA sequencing data Pollina et al. 27 GEO ID# GSE175965 Yap et al., (2021) RNA sequencing data Yap et al. 28 GEO ID# GSE158843 Single nuclear RNA sequencing data This paper GEO accession# GSE315059 Yap et al., (2021) RNA sequencing data Yap et al. 28 GEO ID# GSE158843 Experimental models: Organisms/strains Mouse:C57Bl/6J The Jackson Laboratory Cat# JAX:000664 Mouse:B6.Tnfrsf12a tm1(KO)Biogen Dr. Linda Burkly, Biogen NA mouse:B6.129S4(CG)-Bmal1 tm1Weit /J The Jackson Laboratory Cat# JAX:007668 Oligonucleotides gaaggtaccggatccgccacGAT GGTGAGCAAGGGCGAG Millipore Sigma NA tatcgataagcttgatatcg CAGCGAGTCAGTGAGCGAG Millipore Sigma NA ACAAAAGCTAGCgccaccAT GTACCCATACGATGTTCCAGAT TACGCTAtggcttcggcttggccgc ggtctctgccgcagatcctcgtg ttgggattcggcttggtgttgatgcgcgccg cggccggggagcaagcaccaggcacctc cccatgctctagcggcagctcctggagcgcg gacctcgacaagtgcatggactgcgcttctt gtccagcgcgaccacacagcgac ttctgcctgggatgcgccgcagcacctcctgcccac ttcaggctactgtggcccattctg gggggcgctcttagtctggtcctggttttgg cgctggtttctagtttcctggtctggagaagatgc cgccggagagaaaagtttactacccccat agaggagactggtggagagggctgcc caggtgtggcactgatccagtgaccattaacgcgc gaccagcttgatatcgaattggcccct ctccctcccccccccctaacgtta ctggccgaagccgcttggaataa ggccggtgtgcgtttg tctatatgttattttccaccatattgc cgtcttttggcaatgtgagggcccggaa acctggccctgtcttcttgacgagcattcctaggg gtctttcccctctcgccaaaggaatg caaggtctgttgaatgtcgtgaagg aagcagttcctctggaagcttcttga agacaaacaacgt ctgtagcgaccctttgcaggcagcg gaaccccccacctggcgacaggt gcctctgcggccaaaagccacgtg tataagatacacctgcaa aggcggcacaaccccagtgcca cgttgtgagttggatagttgtggaaaga gtcaaatggctctcctcaagcgtatt caacaaggggctga aggatgcccagaaggtaccccattg tatgggatctgatctggggcctcggtaca catgctttacatgtgtttagtcgaggttaaaaaaac gtctaggccccccgaaccacggggac gtggttttcctttgaaaaacacgatgataat atggccacaaccatggtgagcaagggcgaggag ctgttcaccggggtggtgcccatcc GeneScript NA (Continued on next page) Cell Reports 45, 116926, February 24, 2026 19
Gene Expression:Article Title: Fn14 is an activity-dependent, Bmal1-regulated cytokine receptor that induces rod-like microglia and restricts neuronal activity in vivo.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken anti Iba1 Synaptic Systems Cat# 234009; RRID: AB_2891282 Rabbit anti vGluT1 Invitrogen Cat# 48-2400; RRID: AB_2533843 Mouse anti vGaT Synaptic Systems Cat# 131001; AB_2315584 Alexafluor 488 goat anti rabbit Abcam Cat# 150077; RRID: AB_2630356 Alexafluor 555 rabbit anti goat Thermofisher Cat# A21428; RRID: AB_2535849 Alexafluor 488 chicken anti rabbit Synaptic Systems Rabbit anti Fn14 Cell Signaling Cat# 4403s; RRID: AB_10693941 Rabbit anti HA Cell Signaling Cat# 3724; RRID: AB_1549585 Rabbit anti vGluT1 Invitrogen Cat# 48-2400; RRID: AB_2533843 Alexafluor 555 donkey anti rabbit Invitrogen Cat# A31572; RRID: AB_162543 Alexafluor 647 donkey anti chicken Jackson Immuno Research Cat# 703-605-155; RRID: AB_2340379 Chicken anti Homer1b/c Synaptic Systems Cat# 160026; RRID: AB_2713982 Bacterial and virus strains pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 James M. Wilson Addgene 100834 eBFP2 (eBFP2)_CD Iverson et al 2016 Addgene 66034 pAAV-hSyn-EGFP Bryan Roth Addgene 50465 AAV9-hSyn-eBFP2 Made in house NA AAV9-hSyn-HA-Fn14-IRES-eGFP Made in house NA pENN.AAV.hSyn.HI.eGFPCre.WPRE.SV40 James M. Wilson Addgene 105540 Chemicals, peptides, and recombinant proteins Kainate Sigma-Aldrich Cat# K0250-50MG EcoRI-HF New England BioLabs Cat# NEB:R3101s BamHI-HF New England BioLabs Cat# NEB:R3136s Gibson reaction New England BioLabs Cat# NEB: E2621S T4 ligase New England BioLabs Cat# NEB:M0202S Pentylenetetrazole Sigma-Aldrich Cat# P6500 ViaStain AOPI Nexcelom Cat# CS2-0106-5mL RIPA lysis buffer Thermoscientific Cat# 89900 Protease inhibitor cocktail Thermoscientific Cat# 78429 Phosphotase inhibitor cocktail Thermoscientific Cat# 78428 Sodium n-dodecyl sulfate (SDS) Thermoscientific Cat# 28364 1M Tris-HCI, pH 8.0 Thermoscientific Cat# 15568025 NuPAGE TM LDS Sample Buffer (4X) Thermoscientific Cat# NP0007 XT MES Running Buffer Bio-Rad Cat# 1610789 Sodium Deoxycholate Detergent Thermoscientific Cat# 89904 Critical commercial assays RNAscope multiplex fluorescent V2 assay Advanced Cell Diagnostics [ACD], Biotechne Cat# 323110 Quantitative multiplexed detection of cytokines, chemokines, and growth factors Eve Technologies Corporation NA GEM-X Universal Gene Expression v4 kit 10X Genomics Cat# 1000691 TransAM® AP-1 Family Activ Motif Cat# 44296 (Continued on next page) 18 Cell Reports 45, 116926, February 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Pollina et al., (2023) RNA sequencing data Pollina et al. 27 GEO ID# GSE175965 Yap et al., (2021) RNA sequencing data Yap et al. 28 GEO ID# GSE158843 Single nuclear RNA sequencing data This paper GEO accession# GSE315059 Yap et al., (2021) RNA sequencing data Yap et al. 28 GEO ID# GSE158843 Experimental models: Organisms/strains Mouse:C57Bl/6J The Jackson Laboratory Cat# JAX:000664 Mouse:B6.Tnfrsf12a tm1(KO)Biogen Dr. Linda Burkly, Biogen NA mouse:B6.129S4(CG)-Bmal1 tm1Weit /J The Jackson Laboratory Cat# JAX:007668 Oligonucleotides gaaggtaccggatccgccacGAT GGTGAGCAAGGGCGAG Millipore Sigma NA tatcgataagcttgatatcg CAGCGAGTCAGTGAGCGAG Millipore Sigma NA ACAAAAGCTAGCgccaccAT GTACCCATACGATGTTCCAGAT TACGCTAtggcttcggcttggccgc ggtctctgccgcagatcctcgtg ttgggattcggcttggtgttgatgcgcgccg cggccggggagcaagcaccaggcacctc cccatgctctagcggcagctcctggagcgcg gacctcgacaagtgcatggactgcgcttctt gtccagcgcgaccacacagcgac ttctgcctgggatgcgccgcagcacctcctgcccac ttcaggctactgtggcccattctg gggggcgctcttagtctggtcctggttttgg cgctggtttctagtttcctggtctggagaagatgc cgccggagagaaaagtttactacccccat agaggagactggtggagagggctgcc caggtgtggcactgatccagtgaccattaacgcgc gaccagcttgatatcgaattggcccct ctccctcccccccccctaacgtta ctggccgaagccgcttggaataa ggccggtgtgcgtttg tctatatgttattttccaccatattgc cgtcttttggcaatgtgagggcccggaa acctggccctgtcttcttgacgagcattcctaggg gtctttcccctctcgccaaaggaatg caaggtctgttgaatgtcgtgaagg aagcagttcctctggaagcttcttga agacaaacaacgt ctgtagcgaccctttgcaggcagcg gaaccccccacctggcgacaggt gcctctgcggccaaaagccacgtg tataagatacacctgcaa aggcggcacaaccccagtgcca cgttgtgagttggatagttgtggaaaga gtcaaatggctctcctcaagcgtatt caacaaggggctga aggatgcccagaaggtaccccattg tatgggatctgatctggggcctcggtaca catgctttacatgtgtttagtcgaggttaaaaaaac gtctaggccccccgaaccacggggac gtggttttcctttgaaaaacacgatgataat atggccacaaccatggtgagcaagggcgaggag ctgttcaccggggtggtgcccatcc GeneScript NA (Continued on next page) Cell Reports 45, 116926, February 24, 2026 19
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